Vol. 19 No. 4-2 (2015): Cell Technologies in Cardiology (Special Issue)
EXPERIMENTAL STUDIES

Viability modulation of cells immobilized in porous TiNi-based alloy scaffold under infrared and ultraviolet radiation

PDF (Русский)
  • S. Gyunter,
  • O. Kokorev,
  • V. Khodorenko,
  • G. Dambaev
S. Gyunter
Tomsk National Research State University, 17 19 Gv. Divizii St., 634045 Tomsk, Russian Federation
O. Kokorev
Tomsk National Research State University, 17 19 Gv. Divizii St., 634045 Tomsk, Russian Federation
V. Khodorenko
Tomsk National Research State University, 17 19 Gv. Divizii St., 634045 Tomsk, Russian Federation
G. Dambaev
Siberian State Medical University, 2 Moskovskiy Trakt St., 634050 Tomsk, Russian Federation
DOI: https://doi.org/10.21688/1681-3472-2015-4-2-62-68

Published 2016-01-14

Keywords

  • scaffold,
  • extracellular matrix,
  • porous TiNi scaffold,
  • IR,
  • UV radiation

How to Cite

Gyunter, S., Kokorev, O., Khodorenko, V., & Dambaev, G. (2016). Viability modulation of cells immobilized in porous TiNi-based alloy scaffold under infrared and ultraviolet radiation. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(4-2), 62–68. https://doi.org/10.21688/1681-3472-2015-4-2-62-68

Copyright (c) 2016 Gyunter S.V., Kokorev O.V., Khodorenko V.N., Dambaev G.Ts.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Cellular response to electromagnetic radiation depends on many factors including the microenvironment of cell. Different cells (spleen, bone marrow, and Ehrlich's adenocarcinoma), respond to the studied types of low-intensity radiation. Exposure to infrared (IR) and ultraviolet (UV) radiation of low intensity leads to significant change in the number of viable cells. Effects of low-intensity infrared (IR) and ultraviolet (UV) radiation on the number of viable cells were evaluated against the control group in which cells were exposed to natural daylight. The results showed that IR irradiation led to a 4.6-, 2.5-, and 1.3-fold increase in viable Ehrlich tumor, bone marrow, and spleen cells, respectively, while UV exposure led to a 3.9-, 1.5-, and 1.2-fold increase, respectively. The data show that the extracellular environment of bone marrow, tumor and spleen cell populations affects their viability and proliferative potency in porous TiNi-based scaffolds. IR- and UV irradiation of cell cultures immobilized in the scaffold affects the cell viability in populations of bone marrow, tumor, and spleen cells. In case of IR irradiation, cell viability was significantly improved, at the same time UV irradiation suppressed cell proliferation activity. The effect of IR irradiation can be used to resuscitate the cell area. The effect of UV irradiation can be used to destroy residual tumor lesions or other pathological cell populations.
PDF (Русский)

References

  1. Badylak S.F., Taylor D., Uygun K. Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds // Annu. Rev. Biomed. Eng. 2011. Vol. 13. P. 27–53.
  2. Atala A. Engineering tissues, organs and cells // J. Tissue Eng. Regen. Med. 2007. Vol. 1. P. 83–96.
  3. L’Heureux N., McAllister T.N., de la Fuente L.M. Tissue-engineered blood vessel for adult arterial revascularization // New Engl. J. Med. 2007. Vol. 357. P. 1451–3.
  4. Baptista P.M., Siddiqui M.M., Lozier G., Rodriguez S.R., Atala A., Soker S. The use of whole organ decellularization for the generation of a vascularized liver organoid // Hepatology. 2010. Vol. 53. P. 604–17.
  5. Badylak S.F. Regenerative medicine and developmental biology: the role of the extracellular matrix // Anat. Rec. 2005. Vol. 287 B. P. 36–41.
  6. Ott H.C., Matthiesen T.S., Goh S.-K., Black L.D., Kren S.M., Netoff T.I., Taylor D.A. Perfusion-decellularized matrix: using nature’s platform to engineer a bioartificial heart // Nature Med. 2008. Vol. 14. P. 213–21.
  7. Taylor D.A. From stem cells and cadaveric matrix to engineered organs // Curr. Opin. Biotechnol. 2009. Vol. 20. P. 598–605.
  8. Бенч Л., Джонс Д. Биоматериалы, искусственные органы и инжиниринг тканей. М.: Техносфера, 2007. 304 с.
  9. Orlando G., Wood K,J,, Stratta R,J,, Yoo J,J,, Atala A,, Soker S. Regenerative medicine and organ transplantation: past, present, and future // Transplantation. 2011. Vol. 91. P. 1310–7.
  10. Orlando G., Baptista P., Birchall M., De Coppi P., Farney A., Guimaraes-Souza N.K., Opara E., Rogers J., Seliktar D., Shapira-Schweitzer K., Stratta R.J., Atala A., Wood K.J., Soker S. Regenerative medicine as applied to solid organ transplantation: current status and future challenges // Transpl. Int. 2011. Vol. 24. P. 223–32.
  11. Кокорев О.В., Дамбаев Г.Ц., Ходоренко В.Н., Гюнтер В.Э. Применение пористо-проницаемых инкубаторов из никелида титана в качестве носителей клеточных культур // Клеточная трансплантология и тканевая инженерия. 2010. Т. 5. № 4. С. 31–37.
  12. Медицинские материалы и имплантаты с памятью формы: В 14 томах // Под ред. В.Э. Гюнтера. Медицинские материалы с памятью формы. Т. 1. Томск: Изд-во МИЦ, 2011. 534 с.
  13. Медицинские материалы и имплантаты с памятью формы: В 14 томах // Под ред. В.Э. Гюнтера. Имплантаты с памятью формы в хирургии. Т. 11. Томск: Изд-во МИЦ, 2012. 398 с.
  14. Kokorev O.V., Hodorenko V.N., Chekalkin T.L., Dambaev G.Ts., Gunther V.E. Porous TINI-based alloy scaffold for cell tissue engineering // Journal of Advanced Scientific Research. 2014. Vol. 5. № 3. P. 01–06.
  15. Almine J.F., Bax D.V., Mithieux S.M., Nivison-Smith L., Rnjak J., Waterhouse A., Wise S.G., Weiss A.S. Elastin-based materials // Chem. Soc. Rev. 2010. Vol. 39. P. 3371–9.
  16. Kanematsu A., Yamamoto S., Ozeki M., Noguchi T., Kanatani I., Ogawa O., Tabata Y. Collagenous matrices as release carriers of exogenous growth factors // Biomaterials. 2004. Vol. 25. P. 4513–20.
  17. Price A.P., England K.A., Matson A.M., Blazar B.R., Panoskaltsis-Mortari A. Development of a decellularized lung bioreactor system for bioengineering the lung: the matrix reloaded // Tissue Eng. A. 2010. Vol. 16. P. 2581–91.
  18. Альбертс Б., Брейт Д. Молекулярная биология клетки. Т. 1. М.: Мир, 1994. 350 с.
  19. Тучин В.В. Оптическая биомедицинская диагностика. Т. 2. М., 2007. 367 c.
  20. Ремизов А.Н. Медицинская и биологическая физика. М.: Высш. школа, 1996. 608 с.
  21. Исаков В.Л. Основные вопросы разработки методических рекомендаций по лазерной медицине // Применение лазеров в биологии и медицине. К., 1995. С. 7–20.
  22. Антонов В.Ф., Черныш А.М, Пасечник В.И. Биофизика. М.: Физика, 2000. 154 с.
  23. Вайль Н.С. Инфракрасные лучи в клинической диагностике и медико-биологических исследованиях. М.: Медицина, 1996. 278 с.
  24. Мейер А., Зейтц Э. Ультрафиолетовое излучение. М.: Науч. лит-ра, 1989. 574 с.
  25. Патент RU № 2438699. Способ изготовления вакцины для лечения адэнокарциномы Эрлиха в эксперименте // С.В. Гюнтер, О.В. Кокорев. 7 с.