Vol. 19 No. 1 (2015)
FUNDAMENTAL RESEARCH

Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure

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  • N. Bondarenko,
  • A. Lykov,
  • I. Kim,
  • O. Poveshchenko,
  • A. Poveshchenko,
  • Ye. Pokushalov,
  • A. Romanov,
  • A. Karaskov,
  • V. Konenkov
N. Bondarenko
Institute of Clinical and Experimental Lymphology; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
A. Lykov
Institute of Clinical and Experimental Lymphology; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
I. Kim
Institute of Clinical and Experimental Lymphology; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
O. Poveshchenko
Institute of Clinical and Experimental Lymphology; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
A. Poveshchenko
Institute of Clinical and Experimental Lymphology; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
Ye. Pokushalov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
A. Romanov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
A. Karaskov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
Bio
V. Konenkov
Institute of Clinical and Experimental Lymphology; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology
DOI: https://doi.org/10.21688/1681-3472-2015-1-90-94

Published 2015-10-10

Keywords

  • CRYOPRESERVATION,
  • MONONUCLEAR CELLS,
  • ENDOTHELIAL PROGENITOR CELLS,
  • CHRONIC HEART FAILURE

How to Cite

Bondarenko, N., Lykov, A., Kim, I., Poveshchenko, O., Poveshchenko, A., Pokushalov, Y., Romanov, A., Karaskov, A., & Konenkov, V. (2015). Influence of prolonged cryopreservation on the number and functional activity of stem/progenitor cells derived after G-CSF mobilization from patients with chronic heart failure. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(1), 90–94. https://doi.org/10.21688/1681-3472-2015-1-90-94

Copyright (c) 2015 Bondarenko N.A., Lykov A.P., Kim I.I., Poveshchenko O.V., Poveshchenko A.F., Pokushalov Ye.A., Romanov A.B., Karaskov A.M., Konenkov V.I.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objectives and methods. It is shown that long-term cryopreservation for 2 and 4 years leads to a reduction in the number and viability of the mononuclear cells obtained after mobilization granulocyte colony-stimulating factor (G-CSF) from patients with chronic heart failure. In addition, during prolonged cryopreservation decreased amount of content endothelial progenitor cells of the mononuclear cells from patients with chronic heart failure.
Results. It was established that in cryopreservation for 2 and 4 years leads to a decreased proliferative activity of the mononuclear cells mobilized by G-CSF, both spontaneous and mitogen stimulated, increase cell number in the stage of apoptosis. The findings suggest that the negative impact on the long cryopreservation mononuclear cells obtained after G-CSF mobilization and effective cell therapy for heart failure patients need to use cells in the cryopreservation of not more than 2 years.

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