Vol. 27 No. 1 (2023)
EXPERIMENTAL STUDIES

Evaluation of the original sealant with antibacterial effect for synthetic vascular grafts

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  • A.A. Shadanov,
  • I.Yu. Zhuravleva,
  • L.M. Samoylova,
  • T.P. Timchenko,
  • S.V. Vladimirov,
  • E.V. Karpova,
  • N.E. Luchnikov,
  • D.A. Sirota,
  • A.G. Edemskiy,
  • A.V. Bogachev-Prokofiev,
  • A.M. Chernyavskiy
A.A. Shadanov
Meshalkin National Medical Research Center
Bio
I.Yu. Zhuravleva
Meshalkin National Medical Research Center
L.M. Samoylova
Meshalkin National Medical Research Center
T.P. Timchenko
Meshalkin National Medical Research Center
S.V. Vladimirov
Meshalkin National Medical Research Center
E.V. Karpova
N.N. Vorozhtsov Novosibirsk Institute of Organic Chemistry
N.E. Luchnikov
Novosibirsk State Medical University
D.A. Sirota
Meshalkin National Medical Research Center
A.G. Edemskiy
Meshalkin National Medical Research Center
A.V. Bogachev-Prokofiev
Meshalkin National Medical Research Center
A.M. Chernyavskiy
Meshalkin National Medical Research Center
Effectiveness of grafts containing
DOI: https://doi.org/10.21688/1681-3472-2023-1-38-46

Published 2023-03-30

Keywords

  • Gelatin,
  • Permeability,
  • Polyethylene Terephthalates,
  • Vancomycin

How to Cite

Shadanov, A., Zhuravleva, I., Samoylova, L., Timchenko, T., Vladimirov, S., Karpova, E., Luchnikov, N., Sirota, D., Edemskiy, A., Bogachev-Prokofiev, A., & Chernyavskiy, A. (2023). Evaluation of the original sealant with antibacterial effect for synthetic vascular grafts. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 27(1), 38–46. https://doi.org/10.21688/1681-3472-2023-1-38-46

Copyright (c) 2023 Shadanov A.A., Zhuravleva I.Yu., Samoylova L.M., Timchenko T.P., Vladimirov S.V., Karpova E.V., Luchnikov N.E., Sirota D.A., Edemskiy A.G., Bogachev-Prokofiev A.V., Chernyavskiy A.M.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background: Vascular graft infection is a severe cardiovascular complication with high mortality rates. Antibacterial vascular graft coatings are widely used and continue to be improved.
Objective: To develop a sealant with antimicrobial effect for Dacron vascular grafts and experimentally test the coating properties.
Methods: We used 2 types of vascular grafts: graft No. 1 (plain weave) with initial water permeability of 78.8 ± 2.7 mL/cm2/min and graft No. 2 (2/1 twill + 6/4 satin weave) with water permeability of 549.8 ± 20.7 mL/cm2/min. We developed an original gelatin impregnation technique, tested it using a scanning electron microscopy, and measured water permeability and kink radius. We evaluated the antibacterial activity of vancomycin and gelatin impregnation by the disk diffusion test using media with Staphylococcus aureus ATCC 29213 and Enterococcus faecalis ATCC 29212 and measured inhibition zones after 24-hour incubation. We used samples cut out of grafts with vancomycin-containing (experimental group) and no vancomycin-containing (control group) impregnation.
Results: The gelatin coating of the samples is visualized as a thin even film and provides zero water permeability. On the negative side, it increased stiffness and the kink radius. Thanks to vancomycin added to the coating, bacterial growth significantly inhibits: the mean diameter of the growth inhibition zone for Staphylococcus aureus and Enterococcus faecalis was 15.60 ± 0.65 and 14.40 ± 0.66 mm, respectively. The grafts untreated with vancomycin showed no growth inhibition zones.
Conclusion: Vascular graft treatment with gelatin impregnation and vancomycin solution is a simple measure to prevent the growth of gram-positive bacteria on vascular grafts.

Received 14 November 2022. Revised 15 December 2022. Accepted 19 December 2022.

Funding: The work was supported by Russian Science Foundation and the government of the Novosibirsk region (project No. 22-15-20005).

Conflict of interest: The authors declare no conflict of interest.

Contribution of the authors
Conception and study design: I.Yu. Zhuravleva, A.A. Shadanov, D.A. Sirota
Data collection and analysis: A.A. Shadanov, L.M. Samoylova, S.V. Vladimirov, T.P. Timchenko, N.E. Luchnikov, E.V. Karpova
Statistical analysis: A.A. Shadanov
Drafting the article: A.A. Shadanov, L.M. Samoylova, T.P. Timchenko, A.G. Edemskiy
Critical revision of the article: D.A. Sirota, I.Yu. Zhuravleva, A.V. Bogachev-Prokofiev, A.M. Chernyavskiy
Final approval of the version to be published: A.A. Shadanov, I.Yu. Zhuravleva, L.M. Samoylova, T.P. Timchenko, S.V. Vladimirov, E.V. Karpova, N.E. Luchnikov, D.A. Sirota, A.G. Edemskiy, A.V. Bogachev-Prokofiev, A.M. Chernyavskiy

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