Vol. 23 No. 3 (2019)
Endovascular Surgery

Investigation of the cytotoxic effects of magnesium alloys on cell cultures

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  • T. Frolova,
  • A. Boykov,
  • A. Tarkova,
  • K. Orishchenko,
  • A. Prokhorikhin,
  • D. Malaev,
  • O. Sinicyna,
  • V. Baystrukov,
  • E. Kretov,
  • M. Pryamov,
  • D. Merson,
  • A. Vinogradov
T. Frolova
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Novosibirsk State University, Novosibirsk
A. Boykov
Meshalkin National Medical Research Center, Novosibirsk
Bio
A. Tarkova
Meshalkin National Medical Research Center, Novosibirsk
K. Orishchenko
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk
A. Prokhorikhin
Meshalkin National Medical Research Center, Novosibirsk
D. Malaev
Meshalkin National Medical Research Center, Novosibirsk
O. Sinicyna
Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Novosibirsk State University, Novosibirsk
V. Baystrukov
Meshalkin National Medical Research Center, Novosibirsk
E. Kretov
Meshalkin National Medical Research Center, Novosibirsk
M. Pryamov
Meshalkin National Medical Research Center, Novosibirsk
D. Merson
Togliatti State University, Togliatti
A. Vinogradov
Togliatti State University, Togliatti
DOI: https://doi.org/10.21688/1681-3472-2019-3-22-29

Published 2019-11-27

Keywords

  • biocompatibility,
  • biodegradable stents,
  • coronary artery disease,
  • endovascular surgery,
  • magnesium alloys,
  • peripheral artery disease
    • ...More
    Less

How to Cite

Frolova, T., Boykov, A., Tarkova, A., Orishchenko, K., Prokhorikhin, A., Malaev, D., Sinicyna, O., Baystrukov, V., Kretov, E., Pryamov, M., Merson, D., & Vinogradov, A. (2019). Investigation of the cytotoxic effects of magnesium alloys on cell cultures. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 23(3), 22–29. https://doi.org/10.21688/1681-3472-2019-3-22-29

Copyright (c) 2019 Frolova T. S., Boykov A. A., Tarkova A. R., Orishchenko K. E., Prokhorikhin A. A., Malaev D. U., Sinicyna O. I., Baystrukov V. I., Kretov E. I., Pryamov M. V., Merson D. L., Vinogradov A. Yu.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Background. Metallic stent implantation is associated with an increased risk of adverse events, such as prolonged endothelial dysfunction, constant traumatisation of the vessel wall, chronic local inflammation and thrombosis. Thus, the development and manufacturing of bioresorbable stents, which maintain the required support during the vessel healing period and completely dissolve without any side effects, is highly relevant. At present, magnesium alloys are regarded the most applicable for this purpose due to their low corrosion resistance and high biocompatibility.
Aim. To assess the cytotoxicity of different magnesium alloys in vitro.
Methods. Using strain tempering, seven samples with different yield stress levels were produced: sample 1, MgZnZr (ZK60) 310 МPа; sample 2, MgZnCa (ZX10) 60 МPа; sample 3, MgZnCa (ZX40) 130 МPа; sample 4, MgYZn (WZ31) 300 МPа; sample 5, MgYZn (WZ31) 275 МPа; sample 6, MgYZn (WZ20) 340 МPа and sample 7, MgZnZr (ZK60) 180 МPа. The samples were incubated in a culture medium to obtain the extract, which was further tested on immortalised human fibroblasts. The cytotoxicity of the obtained extract was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, optical microscopy and pH test.
Results. In MTT assay, sample 7 was significantly cytotoxic (mean cell survival: 48% [2% ± 1%]), whereas sample 5 was slightly cytotoxic (mean cell survival: 81% [4% ± 14%]). In optical microscopy, the same samples showed the lowest cell density. In flow cytometry, the number of necrotic cells significantly increased in sample 7 (8.25%) and only slightly increased in samples 1 and 5 (3.449% and 3.626%, respectively). Furthermore, samples 5 and 7 showed the highest medium pH.
Conclusion. The composition and strain tempering method magnesium alloys are directly correlated with the degree of cell necrosis, change in morphology and medium pH in vitro.

Received 7 June 2019. Revised 28 October 2019. Accepted 6 November 2019.

Funding: The work is supported by the Ministry of Science of Russian Federation (project RFMEFI58317X0070).

Conflict of interest: Authors declare no conflict of interest.

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