Vol. 16 No. 2 (2012)
MORPHOLOGY AND PATHOMORPHOLOGY

Stimulation of angiogenesis in rat tissues after administration of mesenchymal stem cells near thrombosed veins

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  • I. Mayborodin,
  • V. Morozov,
  • Ya. Novikova,
  • V. Matveeva,
  • L. Artemeva,
  • A. Matveev,
  • S. Khomenyuk,
  • S. Marchukov
I. Mayborodin
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
V. Morozov
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
Ya. Novikova
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
V. Matveeva
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
L. Artemeva
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
A. Matveev
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
S. Khomenyuk
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
S. Marchukov
Institute of Chemical Biology and Fundamental Medicine SB RAS
Bio
DOI: https://doi.org/10.21688/1681-3472-2012-2-81-86

Published 2012-07-03

Keywords

  • MULTIPOTENT MESENCHYMAL STEM CELLS,
  • VENOUS THROMBOSIS,
  • RECANALIZATION OF BLOOD CLOTS,
  • ANGIOGENESIS IN GRANULATIONS,
  • RESTORATION OF BLOOD FLOW

How to Cite

Mayborodin, I., Morozov, V., Novikova, Y., Matveeva, V., Artemeva, L., Matveev, A., Khomenyuk, S., & Marchukov, S. (2012). Stimulation of angiogenesis in rat tissues after administration of mesenchymal stem cells near thrombosed veins. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 16(2), 81–86. https://doi.org/10.21688/1681-3472-2012-2-81-86

Copyright (c) 2012 Mayborodin I.V., Morozov V.V., Novikova Ya.V., Matveeva V.A., Artemeva L.V., Matveev A.L., Khomenyuk S.V., Marchukov S.V.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The effect of introduction of autologous multipotent mesenchymal stem cells of bone marrow origin (MSC) with a GFP gene and additionally marked by DAPI dye for cellular nuclei into the area close to the thrombosed vein of a rat's hind leg has been studied by using methods of luminescent microscopy. It has been revealed that MSC take are involved in the development of granulations in a place of surgical intervention performed at the time of thrombosis modeling. Restoration of the blood flow in the thrombosed main vein always follows as a result of thrombolysis. No signs of MSC embedded in the walls of the thrombosed vessels have been noticed. Recanalization of blood clots and formation of collaterals hasn't been observed. When modeling thrombosis by introducing athrombin and ligating the main vein, the latter's small tributaries also get thrombosed. Restoration of blood flow in the tributaries occurs with involvement of embedded MSC either through recanalization of blood clots or through obliteration of thrombosed vessels and formation of new ones. Accumulated MSC and the structures generated with their participation are forced out by the organism-recipient's own cells.
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