Vol. 19 No. 4-2 (2015): Cell Technologies in Cardiology (Special Issue)
EXPERIMENTAL STUDIES

Angiogenic potential of rat cardiac stem cells and bone marrow mesenchymal stromal cells

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  • S. Pavlova,
  • I. Rozanova,
  • E. Chepeleva,
  • A. Malakhova,
  • A. Lykov,
  • E. Pokushalov,
  • S. Zakiyan
S. Pavlova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
I. Rozanova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
E. Chepeleva
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
A. Malakhova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
A. Lykov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; Research Institute of Clinical and Experimental Lymphology, 2 Timakova St., 630060 Novosibirsk, Russian Federation
E. Pokushalov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
S. Zakiyan
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
DOI: https://doi.org/10.21688/1681-3472-2015-4-2-77-84

Published 2016-01-14

Keywords

  • cardiac stem cells,
  • bone marrow derived mesenchimal stromal cells,
  • cardiomyocyte,
  • endotheliocyte,
  • pericyte

How to Cite

Pavlova, S., Rozanova, I., Chepeleva, E., Malakhova, A., Lykov, A., Pokushalov, E., & Zakiyan, S. (2016). Angiogenic potential of rat cardiac stem cells and bone marrow mesenchymal stromal cells. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(4-2), 77–84. https://doi.org/10.21688/1681-3472-2015-4-2-77-84

Copyright (c) 2016 Pavlova S.V., Rozanova I.A., Chepeleva E.V., Malakhova A.A., Lykov A.P., Pokushalov E.A., Zakiyan S.M.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Cell therapy is a promising approach in the treatment of ischaemic myocardial damage. Multiple research data show that c-kit+ cardiac stem cells of adult organism as well as mesenchymal stromal bone marrow cells demonstrate paracrine effect in the treatment of ischaemic myocardial damage. Comparative immunofluorescent, flow cytometry and RT-PCR analysis of c-kit positive cardiac stem cells and bone marrow derived mesenchimal stromal cells in rats indicates that both types of cells have similar mesenchimal (CD90, CD29, CD73, vimentin, collagen type I, fibronectin), pericyte (aSMA, NG2, nestin , alkaline phosphatase type I, Sca1), endothelial (CD31, Tie2, vW) markers and express paracrine factors and their receptors (PDGFb / PDGFRb, VEGF /VEGFR2, Ang1 / Tie2, HGF / c-met, TGFb, Sfrp1). Cultivation of CSC and BM-MSC in endothelial medium EGM2 helped to improve the angiogenic potential of the culture, as evidenced by the intense isolectin B4 binding, the formation of capillary-like structures in Matrigel test. We hypothesized that c-kit+ CSC are regional mesenchymal stem cells with features of cardiac pericytes. Moreover, we showed that rat bone marrow derived mesenchimal stromal cells demonstrate angiogenic potential in vitro.

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