Vol. 19 No. 4-2 (2015): Cell Technologies in Cardiology (Special Issue)
EXPERIMENTAL STUDIES

The use of heart-delivered cell culture labeled with luciferase expression for optimisation of the intramyocardial transplantation protocol

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  • E. Milevskaya,
  • A. Nemudryi,
  • E. Chepeleva,
  • A. Malakhova,
  • S. Pavlova,
  • A. Dokuchaeva,
  • D. Sergeevichev,
  • S. Zakiyan
E. Milevskaya
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; Novosibirsk National Research State University, 2 Pirogova St., 630090 Novosibirsk, Russian Federation
A. Nemudryi
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
E. Chepeleva
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
A. Malakhova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
S. Pavlova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
A. Dokuchaeva
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
D. Sergeevichev
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
S. Zakiyan
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
DOI: https://doi.org/10.21688/1681-3472-2015-4-2-69-76

Published 2016-01-14

Keywords

  • heart-delivered cells,
  • cardiac stem cells,
  • fibrin gel,
  • myocardial infarction,
  • bioluminescence

How to Cite

Milevskaya, E., Nemudryi, A., Chepeleva, E., Malakhova, A., Pavlova, S., Dokuchaeva, A., Sergeevichev, D., & Zakiyan, S. (2016). The use of heart-delivered cell culture labeled with luciferase expression for optimisation of the intramyocardial transplantation protocol. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(4-2), 69–76. https://doi.org/10.21688/1681-3472-2015-4-2-69-76

Copyright (c) 2016 Milevskaya E.A., Nemudryi A.A., Chepeleva E.V., Malakhova A.A., Pavlova S.V., Dokuchaeva A.A., Sergeevichev D.S., Zakiyan S.M.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

The use of heart-delivered cell culture (HCC) is one of promising directions of therapy of ischemic heart injury. The success of cell therapy depends of efficiency of cell delivery and its homing to injury area. Application of the phenomenon of bioluminescence for transplanted cells homing monitoring is a very convenient and sparing method which permits to assess a condition of cells dynamically using only one animal. A linear correlation between luciferase activity and cell number was shown. That fact allows to assess HCC transplantation and homing efficiency depending on luciferase activity in cells quantitatively. Different ways of intramyocardial transplantation were compared and it was shown that cell injection in fibrin gel composition promote more effective consolidation of cells in the zone of transplantation.

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