Vol. 19 No. 4-2 (2015): Cell Technologies in Cardiology (Special Issue)
EXPERIMENTAL STUDIES

Correlation of cytokine profile mononuclear and endothelial progenitor cells, obtained in the course of the mobilization of granulocyte colony-stimulating factor in patients with chronic heart failure

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  • O. Poveshchenko,
  • N. Bondarenko,
  • A. Lykov,
  • I. Kim,
  • M. Surovtseva,
  • A. Poveshchenko,
  • E. Pokushalov,
  • A. Romanov,
  • A. Karas'kov,
  • V. Konenkov
O. Poveshchenko
Research Institute of Clinical and Experimental Lymphology, 2 Timakova St., 630060 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
N. Bondarenko
Research Institute of Clinical and Experimental Lymphology, 2 Timakova St., 630060 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
A. Lykov
Research Institute of Clinical and Experimental Lymphology, 2 Timakova St., 630060 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
I. Kim
Research Institute of Clinical and Experimental Lymphology, 2 Timakova St., 630060 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
M. Surovtseva
Research Institute of Clinical and Experimental Lymphology, 2 Timakova St., 630060 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
A. Poveshchenko
Research Institute of Clinical and Experimental Lymphology, 2 Timakova St., 630060 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
E. Pokushalov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
A. Romanov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
A. Karas'kov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
V. Konenkov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
DOI: https://doi.org/10.21688/1681-3472-2015-4-2-55-61

Published 2016-01-14

Keywords

  • cytokines,
  • growth factors,
  • mononuclear cells,
  • endothelial progenitor cells,
  • chronic heart failure

How to Cite

Poveshchenko, O., Bondarenko, N., Lykov, A., Kim, I., Surovtseva, M., Poveshchenko, A., Pokushalov, E., Romanov, A., Karas’kov, A., & Konenkov, V. (2016). Correlation of cytokine profile mononuclear and endothelial progenitor cells, obtained in the course of the mobilization of granulocyte colony-stimulating factor in patients with chronic heart failure. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(4-2), 55–61. https://doi.org/10.21688/1681-3472-2015-4-2-55-61

Copyright (c) 2016 Poveshchenko O.V., Bondarenko N.A., Lykov A.P., Kim I.I., Surovtseva M.A., Poveshchenko A.F., Pokushalov E.A., Romanov A.B., Karas'kov A.M., Konenkov V.I.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: The aim of the study was to evaluate the ability of cytokine peripheral blood mononuclear cells and its correlation with the release of endothelial progenitor cells after mobilization of G-CSF in patients with chronic heart failure.
Methods: Mononuclear cells were obtained from peripheral blood of 35 patients with chronic heart failure before and after mobilization procedures G-CSF (granulocyte colony stimulating factor). Spectrum of cytokine production and growth factors mononuclear cells was evaluated by ELISA in spontaneous conditions and upon stimulation of cells with concanavalin A, lipopolysaccharide, G-CSF, erythropoietin.
Results: A statistically significant increase in the spontaneous production of IL-18, VEGF, Epo and reducing TNF-α production and G-CSF mononuclear cells after mobilization procedures G-CSF. A statistically significant increase in mononuclear cell production of IL-18, VEGF, and G-CSF in response to mitogenic stimulation (Con A) and decrease in production of IL-8 after mobilization procedures G-CSF. In response to an antigenic stimulus (LPS) mononuclear cells were enriched with endothelial progenitor cells from patients with chronic heart failure responded statistically significant increase in the production of IL-18 and G-CSF, and decreased production - TNF-α, as compared to similar products of cytokines and growth factors prior to the procedure to mobilize G-CSF. Proangiogenic cytokines G-CSF or Epo result in a statistically significant increase in the production TNF-α, IL-10, VEGF, and G-CSF mononuclear cells enriched endothelial progenitor cells in patients with chronic heart failure.
Conclusion: Peripheral blood mononuclear cells, endothelial progenitor cells enriched after mobilization of G-CSF, in patients with chronic heart failure produce cytokines and growth factors with proangiogenic effect. Thus, endothelial progenitor cells contribute to the production of cytokines and growth factors such as TNF-α, IL-18, IL-10, Epo, VEGF. Mononuclear cells were obtained after mobilization of G-CSF, can be used to treat chronic heart failure.

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