Vol. 19 No. 4-2 (2015): Cell Technologies in Cardiology (Special Issue)
EXPERIMENTAL STUDIES

Development of cell technologies for design of cell contained vascular grafts

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  • I. Zakharova,
  • M. Zhiven',
  • Sh. Saaya,
  • A. Shevchenko,
  • A. Strunov,
  • L. Ivanova,
  • A. Karpenko,
  • E. Pokushalov,
  • S. Zakiyan
I. Zakharova
The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
M. Zhiven'
The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
Sh. Saaya
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
A. Shevchenko
The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
A. Strunov
The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
L. Ivanova
The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Novosibirsk National Research State University, 2 Pirogova St., 630090 Novosibirsk, Russian Federation
A. Karpenko
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
E. Pokushalov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
S. Zakiyan
The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; Novosibirsk National Research State University, 2 Pirogova St., 630090 Novosibirsk, Russian Federation
DOI: https://doi.org/10.21688/1681-3472-2015-4-2-43-54

Published 2016-01-14

Keywords

  • vascular tissue engineering,
  • endothelium,
  • smooth muscle cells,
  • extracellular matrix

How to Cite

Zakharova, I., Zhiven’, M., Saaya, S., Shevchenko, A., Strunov, A., Ivanova, L., Karpenko, A., Pokushalov, E., & Zakiyan, S. (2016). Development of cell technologies for design of cell contained vascular grafts. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(4-2), 43–54. https://doi.org/10.21688/1681-3472-2015-4-2-43-54

Copyright (c) 2016 Zakharova I.S., Zhiven' M.K., Saaya Sh.B., Shevchenko A.I., Strunov A.A., Ivanova L.N., Karpenko A.A., Pokushalov E.A., Zakiyan S.M.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

A new protocol for production of functional endothelial and mural cells from human cardiac explants has been developed. The endothelial cells are characterized by the presence of mature endothelial markers: CD 31, VE-cadherin and VEGFR2. Functionally they could take up ac-LDL, form tube-like structure in matrigel, contains functional cytoplasmic microvesicles Weibel-Palade bodies, in cytoplasm microvesicles – metabolized form of acetylated low-density lipoprotein, form capillary-like structures in Matrigel and produces extracellular matrix. The smooth muscle cells expressed a specific marker aSMA and produces extracellular matrix. The derived cell populations exhibit functional properties in hindlimb ischemia model. It was shown that the endothelial and the smooth muscle cells retain their specific surface antigens and the ability to produce an extracellular matrix on polycaprolactone and polylactide-co-glycolide surfaces. These cells can be used to develop a vascular tissue-graft.

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