Vol. 19 No. 4-2 (2015): Cell Technologies in Cardiology (Special Issue)
EXPERIMENTAL STUDIES

Cell culture from rat skeletal muscles to be used for cellular therapy of ischemic heart disease

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  • E. Chepeleva,
  • S. Pavlova,
  • A. Malakhova,
  • E. Pokushalov,
  • S. Zakiyan
E. Chepeleva
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
S. Pavlova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
A. Malakhova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
E. Pokushalov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
S. Zakiyan
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
DOI: https://doi.org/10.21688/1681-3472-2015-4-2-28-32

Published 2016-01-14

Keywords

  • skeletal muscle myoblasts,
  • cell culture,
  • cellular therapy

How to Cite

Chepeleva, E., Pavlova, S., Malakhova, A., Pokushalov, E., & Zakiyan, S. (2016). Cell culture from rat skeletal muscles to be used for cellular therapy of ischemic heart disease. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(4-2), 28–32. https://doi.org/10.21688/1681-3472-2015-4-2-28-32

Copyright (c) 2016 Chepeleva E.V., Pavlova S.V., Malakhova A.A., Pokushalov E.A., Zakiyan S.M.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

When treating ischemic heart disease, surgery and drug therapy can be efficiently complemented with cellular therapy. At present, various cell types (embryonic stem cells, mesenchymal stem cells, regional cardiac stem cells, etc.) are studied and the conditions for their generation, cultivation and delivery to the organism are compared to choose the best possible strategy of cellular treatment. Skeletal muscle myoblasts, progenitor cells of myocytes are considered as a promising material for transplantation into peri-infarction tissue. The advantage of these cells lies in the fact that they are easy to be obtained from a donor and that they are capable of differentiating in contractile cells which can improve the myocardial contractile function when engineered in peri-infarction tissue. The study looks at a protocol of deriving cell cultures from rat skeletal muscles and their potential for cardiac myogenic differentiation. Also studied is the impact of a type of culture surface upon the derived cell phenotype.

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References

  1. Roger V.L., Go A.S., Lloyd-Jones D.M. et al. Heart disease and stroke statistics–2011 update: a report from the American Heart Association // Circulation. 2011. Vol. 123. № 4. P. e18–e209.
  2. Rosamond W.D., Chambless L.E., Heiss G., et al. Mosley T.H., Coresh J., Whitsel E., Wagenknecht L., Ni H., Folsom A.R. Twenty-Two Year Trends in Incidence of Myocardial Infarction, CHD Mortality, and Case-Fatality in Four US Communities, 1987 to 2008 // Circulation. 2012. Vol. 125. № 15. P. 1848–1857.
  3. Orn S., Manhenke C., Anand I.S., Squire I., Nagel E., Edvardsen T., Dickstein K. Effect of Left Ventricular Scar Size, Location, and Transmurality on Left Ventricular Remodeling With Healed Myocardial Infarction // Am. J. Cardiol. 2007. Vol. 99. № 8. P. 1109–1114.
  4. White H.D., Aylward P.E., Huang Z. et al. Mortality and Morbidity Remain High Despite Captopril and/or Valsartan Therapy in Elderly Patients With Left Ventricular Systolic Dysfunction, Heart Failure, or Both After Acute Myocardial Infarction: Results From the Valsartan in Acute Myocardial Infarction Trial (VALIANT) // Circulation. 2005. Vol. 112. № 22. P. 3391–3399.
  5. Чернявский А.М., Фомичев А.В., Чернявский М.А., Ларионов П.М., Бондарь В.Ю., Сергеевичев Д.С. Сравнительная характеристика эффективности методов непрямой реваскуляризации миокарда в хирургии ишемической болезни сердца // Патология кровообращения и кардиохирургия. 2013. № 1. С. 15–20.
  6. Laflamme M.A., Murry C.E. Heart regeneration // Nature. 2011. Vol. 473. № 7347. P. 326–335. doi: 10.1038/nature10147.
  7. Taylor D.A., Atkins B.Z., Hungspreugs P., Jones T.R., Reedy M.C., Hutcheson K.A., Glower D.D., Kraus W.E. // Nat. Med. 1998. Vol. 4. № 8. P. 929–933.
  8. Menasché P., Hagège A.A., Vilquin J.T., Desnos M., Abergel E., Pouzet B., Bel A., Sarateanu S., Scorsin M., Schwartz K., Bruneval P., Benbunan M., Marolleau J.P., Duboc D. Autologous skeletal myoblast transplantation for severe postinfarction left ventricular dysfunction // J. Am. Coll. Cardiol. 2003. Vol. 41. № 7. P. 1078–1083.
  9. Menasché P., Alfieri O., Janssens S., McKenna W., Reichenspurner H., Trinquart L., Vilquin J.T., Marolleau J.P., Seymour B., Larghero J., Lake S., Chatellier G., Solomon S., Desnos M., Hagège A.A. // Circulation. 2008. Vol. 117. № 9. P. 1189–1200.
  10. Veltman C.E., Soliman O.I., Geleijnse M.L., Vletter W.B., Smits P.C., ten Cate F.J., Jordaens L.J., Balk A.H., Serruys P.W., Boersma E., van Domburg R.T., van der Giessen W.J. Four-year follow-up of treatment with intramyocardial skeletal myoblasts injection in patients with ischaemic cardiomyopathy // Eur. Heart J. 2008. Vol. 29. № 11. P. 1386–1396.
  11. Leobon B., Garcin I., Menasche P., Vilquin J.T., Audinat E., Charpak S. Myoblasts transplanted into rat infarcted myocardium are functionally isolated from their host // Proc. Natl. Acad. Sci. USA. 2003. Vol. 100. № 13. P. 7808–7811.
  12. Conconi M.T., De Coppi P., Bellini S., Zara G., Sabatti M., Marzaro M., Zanon G.F., Gamba P.G., Parnigotto P.P., Nussdorfer G.G. Homologous muscle acellular matrix seeded with autologous myoblasts as a tissue-engineering approach to abdominal wall-defect repair // Biomaterials. 2005. Vol. 26. № 15. P. 2567–2574.
  13. Tamaki T., Akatsuka A., Ando K., Nakamura Y., Matsuzawa H., Hotta T., Roy R.R., Edgerton V.R. // J. Cell Biol. 2002. Vol. 157. № 4. P. 571–577.
  14. Parker M.H., Seale P., Rudnicki M.A. Looking back to the embryo: defining transcriptional networks in adult myogenesis // Nature Reviews Genetics. 2003. Vol. 4. № 7. P. 497–507.