Vol. 19 No. 4-2 (2015): Cell Technologies in Cardiology (Special Issue)
EXPERIMENTAL STUDIES

Comparative analysis of bone marrow derived mesenchymal stromal cells, cardiac regional stem cells and human skin fibroblasts

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  • S. Pavlova,
  • D. Sergeevichev,
  • E. Chepeleva,
  • V. Kozyreva,
  • A. Malakhova,
  • I. Zakharova,
  • E. Grigor'eva,
  • E. Pokushalov,
  • S. Zakiyan
S. Pavlova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
D. Sergeevichev
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
E. Chepeleva
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
V. Kozyreva
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
A. Malakhova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
I. Zakharova
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
E. Grigor'eva
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation
E. Pokushalov
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation
S. Zakiyan
Academician Ye. Meshalkin Novosibirsk Research Institute of Circulation Pathology, Ministry of Health Care of Russian Federation, 15 Rechkunovskaya St., 630055 Novosibirsk, Russian Federation; The Federal Research Center Institute of Cytology and Genetics, The Siberian Branch of the Russian Federation Academy of Sciences, 10 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Institute of Chemical Biology and Fundamental Medicine, The Siberian Branch of the Russian Federation Academy of Sciences, 8 Lavrentieva Avenue, 630090 Novosibirsk, Russian Federation; Novosibirsk National Research State University, 2 Pirogova St., 630090 Novosibirsk, Russian Federation
DOI: https://doi.org/10.21688/1681-3472-2015-4-2-12-19

Published 2016-01-14

Keywords

  • cardiac stem cells,
  • mesenchymal stem cells,
  • myocardium regeneration

How to Cite

Pavlova, S., Sergeevichev, D., Chepeleva, E., Kozyreva, V., Malakhova, A., Zakharova, I., Grigor’eva, E., Pokushalov, E., & Zakiyan, S. (2016). Comparative analysis of bone marrow derived mesenchymal stromal cells, cardiac regional stem cells and human skin fibroblasts. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 19(4-2), 12–19. https://doi.org/10.21688/1681-3472-2015-4-2-12-19

Copyright (c) 2016 Pavlova S.V., Sergeevichev D.S., Chepeleva E.V., Kozyreva V.S., Malakhova A.A., Zakharova I.S., Grigor'eva E.V., Pokushalov E.A., Zakiyan S.M.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

A characteristic marker of cardiac stem cells (CSC) is a tyrosine kinase receptor c-kit. The cells with the c-kit+ phenotype can be easily isolated and cultivated in vitro. However, only CSC of postnatal animal hearts have the true cardiomyogenic potential. Currently it is being proposed that there are two lineages of c-kit+ cell population in heart. C-kit+ cells capable to differentiate into cardiomyocytes derive from the primary heart field and disappear soon after birth, and иc-kit+ cells of proepicardial origin expressing mesenchymal markers are capable to differentiate into endothelial, mural and fibroblast direction. We have previously described c-kit+ cells isolated from fragments of right atrial appendage, and investigated their angiogenic potential in vitro. In this study, we compared the surface markers of MSCs-BM, CSC and human dermal fibroblasts by flow cytometry and RT-PCR analysis.

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