Vol. 27 No. 4 (2023)
EXPERIMENTAL STUDIES

Long-term normothermic autoperfusion of the cardiopulmonary complex ex vivo as a method of effective graft conditioning: an experimental study

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  • M.O. Zhulkov,
  • A.R. Tarkova,
  • I.S. Zykov,
  • A.G. Makaev,
  • A.V. Protopopov,
  • M.N. Murtazaliev,
  • F.Yu. Kosimov,
  • N.A. Karmadonova,
  • Ya.M. Smirnov,
  • E.E. Kliver,
  • A.M. Volkov,
  • H.A. Agaeva,
  • D.A. Sirota
M.O. Zhulkov
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
A.R. Tarkova
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
I.S. Zykov
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
A.G. Makaev
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
Bio
A.V. Protopopov
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
M.N. Murtazaliev
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
F.Yu. Kosimov
Novosibirsk State Medical University, Ministry of Health of Russian Federation, Novosibirsk
N.A. Karmadonova
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
Ya.M. Smirnov
Novosibirsk State University, Novosibirsk
E.E. Kliver
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
A.M. Volkov
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
H.A. Agaeva
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
D.A. Sirota
Meshalkin National Medical Research Center, Ministry of Health of Russian Federation, Novosibirsk
Experimental design
DOI: https://doi.org/10.21688/1681-3472-2023-4-33-42

Published 2023-12-26

Keywords

  • Bretschneider Cardioplegic Solution,
  • Isolated Heart Preparation,
  • Heart Transplantation,
  • Perfusion,
  • Reperfusion,
  • Swine,
  • Tissue Donors
    • ...More
    Less

How to Cite

Zhulkov, M., Tarkova, A., Zykov, I., Makaev, A., Protopopov, A., Murtazaliev, M., Kosimov, F., Karmadonova, N., Smirnov, Y., Kliver, E., Volkov, A., Agaeva, H., & Sirota, D. (2023). Long-term normothermic autoperfusion of the cardiopulmonary complex ex vivo as a method of effective graft conditioning: an experimental study. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 27(4), 33–42. https://doi.org/10.21688/1681-3472-2023-4-33-42

Copyright (c) 2023 Zhulkov M.O., Tarkova A.R., Zykov I.S., Makaev A.G., Protopopov A.V., Murtazaliev M.N., Kosimov F.Yu., Karmadonova N.A., Smirnov Ya.M., Kliver E.E., Volkov A.M., Agaeva H.A., Sirota D.A.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: To compare the effectiveness of 6-hour normothermic autoperfusion of a heart graft ex vivo with pharmaco-cold preservation using Bretschneider's solution (Custodiol, Dr Franz Köhler Chemie GmbH, Bensheim, Germany).
Methods: Landrace pigs weighing 50 ± 5 kg and aged 4–5 months (n = 10) were selected as a model for a series of acute experiments. Cardiopulmonary conditioning using autoperfusion was conducted for 6 hours on the experimental group (n = 5). On the other hand, the control group underwent a 6-hour pharmaco-cold preservation with Bretschneider solution to recover the heart's pumping function. Graft preservation effectiveness was evaluated by measuring hemodynamic parameters, heartbeat, and myocardial ischemia marker concentrations.
Results: After reperfusion and isolation of the working cardiopulmonary complex, cardiac output was 0.63 [0.37; 0.8] L/min and 0.37 [0.23; 0.37] L/min in the experimental and control groups, respectively (P < .05). The levels of CPK-MB, LDH, troponin-I, and lactate in the coronary sinus blood was significantly higher in the control group.
Conclusion: The study demonstrated significant benefits of normothermic autoperfusion in maintaining the morphofunctional status of the donor heart compared to pharmaco-cold preservation using Bretschneider's solution for 6 hours of ex vivo graft conditioning.

Received 16 July 2023. Revised 8 September 2023. Accepted 11 September 2023.

Funding: The study was carried out within the framework of project No. 23-25-10013 (agreement No. 23-25-10013 dated April 20, 2023 with the Russian Science Foundation, agreement No. р-52 dated April 3, 2023 with the Ministry of Science and Innovation Policy of the Novosibirsk Region).

Conflict of interest: The authors declare no conflict of interest.

Contribution of the authors
Conception and study design: M.O. Zhulkov, D.A. Sirota, I.S. Zykov
Data collection and analysis: M.O. Zhulkov, A.R. Tarkova, I.S. Zykov, A.G. Makaev, A.V. Protopopov, M.N. Murtazaliev, F.Yu. Kosimov, N.A. Karmadonova, Ya.M. Smirnov, E.E. Kliver, A.M. Volkov, H.A. Agaeva, D.A. Sirota
Statistical analysis: M.O. Zhulkov
Drafting the article: M.O. Zhulkov
Critical revision of the article: M.O. Zhulkov, D.A. Sirota, I.S. Zykov
Final approval of the version to be published: M.O. Zhulkov, A.R. Tarkova, I.S. Zykov, A.G. Makaev, A.V. Protopopov, M.N. Murtazaliev, F.Yu. Kosimov, N.A. Karmadonova, Ya.M. Smirnov, E.E. Kliver, A.M. Volkov, H.A. Agaeva, D.A. Sirota

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