Vol. 26 No. 4 (2022)
EXPERIMENTAL STUDIES

Safety of intracoronary iopromide solution for experimental pharmaco-cold ex vivo preservation of donor heart

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  • M.O. Zhulkov,
  • D.A. Sirota,
  • I.S. Zykov,
  • A.K. Sabetov,
  • H.A. Agaeva,
  • A.G. Makaev,
  • D.M. Osintsev,
  • A.E. Kalendarev,
  • A.P. Nadeev,
  • V.E. Kliver,
  • A.R. Tarkova,
  • M.A. Ovchinnikova,
  • A.V. Fomichev,
  • N.A. Karmadonova,
  • D.V. Khomushku
M.O. Zhulkov
Meshalkin National Medical Research Center
D.A. Sirota
Meshalkin National Medical Research Center
I.S. Zykov
Meshalkin National Medical Research Center
A.K. Sabetov
Meshalkin National Medical Research Center
H.A. Agaeva
Meshalkin National Medical Research Center
A.G. Makaev
Meshalkin National Medical Research Center
Bio
D.M. Osintsev
Meshalkin National Medical Research Center
A.E. Kalendarev
Meshalkin National Medical Research Center
A.P. Nadeev
Novosibirsk State Medical University
V.E. Kliver
Novosibirsk State Medical University
A.R. Tarkova
Meshalkin National Medical Research Center
M.A. Ovchinnikova
Meshalkin National Medical Research Center
A.V. Fomichev
Meshalkin National Medical Research Center
N.A. Karmadonova
Meshalkin National Medical Research Center
D.V. Khomushku
Meshalkin National Medical Research Center
DOI: https://doi.org/10.21688/1681-3472-2022-4-42-51

Published 2022-12-29

Keywords

  • Animals,
  • Cardiac Output,
  • Heart Transplantation,
  • Iopromide,
  • Ischemia,
  • Myocytes, Cardiac,
  • Tissue Donors,
  • Reperfusion
    • ...More
    Less

How to Cite

Zhulkov, M., Sirota, D., Zykov, I., Sabetov, A., Agaeva, H., Makaev, A., Osintsev, D., Kalendarev, A., Nadeev, A., Kliver, V., Tarkova, A., Ovchinnikova, M., Fomichev, A., Karmadonova, N., & Khomushku, D. (2022). Safety of intracoronary iopromide solution for experimental pharmaco-cold ex vivo preservation of donor heart. Patologiya Krovoobrashcheniya I Kardiokhirurgiya, 26(4), 42–51. https://doi.org/10.21688/1681-3472-2022-4-42-51

Copyright (c) 2022 Zhulkov M.O., Sirota D.A., Zykov I.S., Sabetov A.K., Agaeva H. A., Makaev A.G., Osintsev D.M., Kalendarev A.E., Nadeev A.P., Kliver V.E., Tarkova A.R., Ovchinnikova M.A., Fomichev A.V., Karmadonova N.A., Khomushku D.V.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Objective: To evaluate the effect of intracoronary iopromide (Ultravist®, Bayer, AG, Leverkusen, Germany) during pharmaco-cold ex vivo preservation of a donor heart on the restoration of heart pumping function and cardiomyocyte metabolism in the early post-transplant period.
Methods: Black-and-white calves aged 3 months were used as an experimental model. In the control group, pharmaco-cold preservation of the donor heart was performed by injecting 2 liters of custodiol (Custodiol®, Dr. Franz Köhler Chemie GmbH, Bensheim, Germany) into the aortic root. The hearts were then stored in an appropriate solution at 0 to 1°C for 2 hours. In the experimental group (n = 6), a solution of iopromide and custodiol were injected into the aortic root at a ratio of 50:50 under a pressure of 70-80 mm Hg for 5 minutes after 120 minutes of preservation. Then the coronary bed was washed with 1 liter of custodiol at a pressure of 40 mm Hg and orthotopic heart transplantation was performed. In the post-transplant period, the parameters of central hemodynamics, myocardial oxygen consumption, and the levels of myocardial ischemia markers (troponin I, creatine phosphokinase-MB, lactate dehydrogenase) were assessed.
Results: Twelve orthotopic heart transplantations were performed during the study. In 120 minutes after the restoration of spontaneous cardiac activity, the level of cardiac output was 5.11 [4.99; 5.41] L/min and 5.77 [4.97; 6.62] L/min (p = 0.0009) in the control and experimental groups, respectively. Changes in the concentration of lactate dehydrogenase, troponin I and lactate in the blood flowing from the coronary sinus were significantly higher in the early reperfusion period. However, no statistically significant difference was observed between the groups (p > 0.05). Myocardial oxygen consumption was significantly reduced at reperfusion. However, the initial values were restored by reperfusion minute 60 without any significant intergroup difference (p > 0.05).
Conclusion: We established the safety of intracoronary iopromide administration for pharmaco-cold ex vivo preservation of the donor heart. Intracoronary administration of iopromide solution does not affect the restoration of the pumping function of the heart or metabolism in cardiomyocytes in the early post-transplant period.

Received 6 May 2022. Revised 14 June 2022. Accepted 11 July 2022.

Funding: The study did not have sponsorship.

Conflict of interest: Authors declare no conflict of interest.

Contribution of the authors
Conception and study design: M.O. Zhulkov, D.A. Sirota, I.S. Zykov
Data collection and analysis: M.O. Zhulkov, I.S. Zykov, A.K. Sabetov, H.A. Agaeva, A.G. Makaev, D.M. Osintsev, A.E. Kalendarev, A.P. Nadeev, V.E. Kliver, A.R. Tarkova, M.A. Ovchinnikova, N.A. Karmadonova, D.V. Khomushku
Statistical analysis: M.O. Zhulkov, A.G. Makaev
Drafting the article: M.O. Zhulkov
Critical revision of the article: M.O. Zhulkov, D.A. Sirota, I.S. Zykov, A.V. Fomichev
Final approval of the version to be published: M.O. Zhulkov, D.A. Sirota, I.S. Zykov, A.K. Sabetov, H.A. Agaeva, A.G. Makaev, D.M. Osintsev, A.E. Kalendarev, A.P. Nadeev, V.E. Kliver, A.R. Tarkova, M.A. Ovchinnikova, A.V. Fomichev, N.A. Karmadonova, D.V. Khomushku

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